Abcam, ab197081, Anti-DDI2 antibody

Size: 100µL
Rabbit Polyclonal DDI2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human DDI2 aa 300 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DDI2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q5TDH0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab197081 was affinity purified using an epitope specific to DDI2 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDI2 also known as DNA-damage inducible 1 homolog 2 is a protein involved in several important cellular processes. It functions as an aspartic protease known for its capability to cleave ubiquitinated substrates. This protein has a molecular mass of approximately 50 kDa. Researchers often find DDI2 expression in various human tissues indicating its potential roles in diverse biological processes.
Biological function summary
DDI2 participates in the proteasome-mediated pathway a significant cellular mechanism that regulates protein degradation to maintain cellular homeostasis. It seems to play a part in the endoplasmic reticulum-associated degradation (ERAD) pathway where misfolded proteins get recognized and directed towards destruction. DDI2's involvement in these pathways suggests its critical function in protein quality control. It also may interact with other proteins that contribute to its protease activity enhancing its regulatory role within the cell.
Pathways
DDI2 functions in the context of the ubiquitin-proteasome system (UPS) a fundamental cellular pathway responsible for protein turnover. Through its protease activity DDI2 intersects with pathways involved in the degradation of damaged or misfolded proteins which connects it to the larger ERAD pathway. Evidence shows its interaction with proteins like p97/VCP which assists in protein degradation and ubiquitin which tags proteins for proteasomal activity highlighting DDI2's place in cellular quality control.
DDI2 is associated with several conditions involving protein homeostasis deficits. Its involvement in lipid metabolism disorders points to a potential role in conditions like Niemann-Pick disease. DDI2 dysfunction may result in the inadequate breakdown of proteins contributing to the pathological accumulation found in disorders related to lipid metabolism. Its interaction with the protein p97/VCP known to be involved in various degenerative diseases highlights the importance of understanding DDI2's mechanistic role in these pathologies.