Abcam, ab197435, Human Chemokine Antibody Array (40 Targets) - Quantitative

Size: 8Test / 22Test / 50Test
ab197435 can be used for quantitative measurement of 40 Human cytokines.
Key facts
Assay type:Quantitative,
Reacts with:Human,
Target:CCL21See target data,
Detection method:Fluorescent,
Assay Platform:Multiplex,
Sample types:Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Product details:
ab197435 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Target names: 6Ckine, Axl, BTC, CCL28, CTACK, CXCL16, ENA-78, Eotaxin-3, GCP-2, GRO, HCC-1, HCC-4, IL-9, IL-17F, IL-18 BPa, IL-28A, IL-29, IL-31, IP-10, I-TAC, LIF, LIGHT, Lymphotactin, MCP-2, MCP-3, MCP-4, MDC, MIF, MIP-3α, MIP-3β, MPIF-1, MSPα, NAP-2, OPN, PARC, PF4, SDF-1α, TARC, TECK, TSLP.
The analytes measured by this kit are split across a single array on the same slide, provided in the kit.
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array.Each antibody is spotted in quadruplicate on each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate (not supplied), allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners (see scanner requirements at ). If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. See below for a schematic protocol, the fluorophore is a dye with equivalent to excitation/ emission to Cy3. The standard is affinity purified.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C