Abcam, ab198154, Alexa Fluor® 647 Anti-NNT antibody [8B4BB10]

Size: 100µL
Mouse Monoclonal NNT antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:8B4BB10,
Isotype:IgG1,
Light chain type:kappa,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nicotinamide nucleotide transhydrogenase (NNT) is an enzyme that plays an important role in cellular redox balance by catalyzing the hydride transfer between NADH and NADP+ across the inner mitochondrial membrane. Also known as NNT in common scientific references the enzyme has a mass of approximately 109 kDa. NNT is primarily expressed in tissues with high metabolic demand such as the liver heart and adrenal glands. Its high expression in the mitochondria reflects its essential role in maintaining efficient energy homeostasis.
Biological function summary
NNT regulates the conversion of NADP+ to NADPH a form that serves as an important reducing agent in cellular mechanisms. The enzyme does not function alone but integrates into the mitochondrial membrane working as a part of a complex with other mitochondrial proteins like ATP synthase and cytochrome oxidase. This role is important for antioxidant defense as NADPH is utilized in pathways that mitigate reactive oxygen species maintaining cellular integrity.
Pathways
NNT is a central component in energy metabolism and detoxification processes. It is intricately involved in the pentose phosphate pathway where it helps generate reducing equivalents in the form of NADPH. Additionally NNT is linked to the oxidative phosphorylation pathway ensuring efficient ATP production. The enzyme's activity is closely associated with proteins such as glutathione reductase and thioredoxin which rely on the reducing power of NADPH provided by NNT for their antioxidant functions.
NNT mutations have been associated with familial glucocorticoid deficiency and metabolic disorders which highlight its importance in endocrine and energy balance. Altered NNT activity has been observed in various cases of hypertension and heart failure. The enzyme's interrelationship with proteins like cortisol which is important in adrenal function and insulin in relation to metabolic processes and regulation indicate its broad influence in both energy homeostasis and redox balance.