Abcam, ab198157, Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein

Size: 50µg
Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:50 μl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl2, 4 mM DTT, 20 μM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hour. Add antibody against methylated K27 residue of histone H3, incubate 1 hour. Then incubate 1 hour with the HRP-labeled secondary antibody, followed by addition of the HRP substrate to produce chemiluminescence.,
Accession:Q15022,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Preservative: 1.53% ImidazoleConstituents: 10% Glycerol (glycerin, glycerine), 0.71% Tris HCl, 0.64% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle