Abcam, ab198468, Recombinant human PARP15 protein

Size: 10µg
Recombinant human PARP15 protein is a Human Fragment protein, in the 221 to 444 aa range, expressed in Escherichia coli, with >55%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>55% SDS-PAGE,
Expression system:Escherichia coli,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.Enzyme reaction is conducted using a PARP15 Assay Kit at room temperature for 30 minutes.,
Accession:Q460N3,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Product details:
ab198468 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PARP15 also known as Poly (ADP-Ribose) Polymerase Family Member 15 is an enzyme that acts by adding ADP-ribose polymers to target proteins a process called ADP-ribosylation. This post-translational modification affects protein function and stability. PARP15 has a molecular mass of approximately 75 kDa. It is mainly expressed in the nucleus of cells but can also be observed in the cytoplasm. Its expression varies in different tissues with notable presence in immune-related tissues such as the spleen and lymph nodes.
Biological function summary
PARP15 plays several roles in modulating cellular stress responses and regulating DNA damage repair. It functions as part of a protein complex that involves other PARP family members. PARP15 interacts with chromatin components influencing transcription and maintaining genomic stability. It has shown involvement in modulating pathways that respond to oxidative stress and toxin exposure suggesting a protective role in cellular defense mechanisms.
Pathways
PARP15 is closely linked with the DNA damage response and repair pathways particularly those associated with homologous recombination. In these pathways it functions alongside proteins like BRCA1 and RAD51 ensuring proper DNA repair. PARP15 also has a connection to cell survival signaling pathways such as those regulated by NF-kB thereby contributing to the integration of stress signals into cellular outcomes.
PARP15 has a relation with cancer and autoimmune conditions. Overexpression or abnormal activity of PARP15 correlates with certain cancers where it can affect cancer cell survival through its influence on DNA repair and stress response pathways. Additionally dysfunction in PARP15 or associated pathways may contribute to autoimmune diseases by affecting cell death and inflammatory signaling. Its interaction with other proteins like PARP1 plays a role in these disease states as alterations in these interactions can impact cellular processes and immune regulation.