Abcam, ab198472, Recombinant human UBE2V1 protein

Size: 100µg
Recombinant human UBE2V1 protein is a Human Full Length protein, in the 2 to 170 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:50 μl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 (0.5 μg), UEV1A or other E2, Biotin-Ubiquitin (0.5 μg) and Smurf2 (2 μg) in 50 mM Tris, pH 7.4) for 30 minutes at 37°C. Add to Streptavidin-coated plates for 30 minutes at 37°C. Incubate with anti-Smurf2, then HRP-labeled secondary antibody and chemiluminescent detection,
Accession:Q13404,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBE2V1 also known as ubiquitin-conjugating enzyme E2 variant 1 acts as an E2-like enzyme facilitating the transfer of ubiquitin molecules to substrate proteins. It is a non-canonical ubiquitin-conjugating enzyme because it lacks the active cysteine residue needed to conjugate ubiquitin directly. The molecular mass of UBE2V1 is approximately 20 kDa. UBE2V1 is highly expressed in various human tissues including the spleen liver and placenta suggesting its broad role in cellular functions.
Biological function summary
UBE2V1 collaborates with UBE2N (also known as Ubc13) to form a heterodimer c. This dimer is involved in the process of K63-linked polyubiquitination which does not target proteins for proteasomal degradation but rather plays an important role in DNA damage tolerance and immune response regulation. UBE2V1 when associated with UBE2N aids in the polyubiquitination of protein substrates allowing for their involvement in regulatory mechanisms that are essential for cellular homeostasis.
Pathways
UBE2V1 serves an important function in the NF-κB signaling pathway and DNA repair processes. In the NF-κB pathway UBE2V1 through its interaction with UBE2N contributes to the activation of TAK1 kinase which ultimately activates the transcription factor NF-κB leading to inflammatory and immune responses. In DNA repair it participates in error-free damage bypass mechanism alongside UBE2N. These pathways highlight the integration of UBE2V1 in cellular processes that manage stress and damage responses.
UBE2V1 associates with cancer and immune disorders. Its role in the NF-κB pathway links it to oncogenic processes as deregulated NF-κB signaling often corresponds with tumorigenesis. In addition abnormalities in UBE2V1’s function may lead to aberrant immune responses as its partnership with UBE2N influences inflammatory reactions. Such dysregulation can contribute to both the initiation and progression of diseases like rheumatoid arthritis and various cancers marking UBE2V1 as a potential target for therapeutic intervention.