Abcam, ab198590, Alexa Fluor® 488 Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker

Size: 100µL
Mouse Monoclonal COX IV antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:10G8D12C12,
Isotype:IgG2a,
Light chain type:kappa,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-ab198590 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab198590 was judged as near homogeneity by SDS PAGE., Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle|Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The COX4 protein also known as Cytochrome c oxidase subunit 4 along with its isoform COX4L2 forms part of the electron transport chain in mitochondria. COX4 has a molecular mass of approximately 20.8 kDa and is found in various tissues especially in high-energy-demand organs like the heart and liver. Both COX4 and COX4L2 belong to the inner mitochondrial membrane and play a role in the enzyme complex Cytochrome c oxidase essential for efficient cellular respiration.
Biological function summary
COX4 and COX4L2 contribute to the terminal stage of the mitochondrial electron transport chain. They form core components of the Cytochrome c oxidase complex also called complex IV which facilitates the transfer of electrons from reduced cytochrome c to oxygen. This process not only aids in the production of water but also helps create the proton gradient used by ATP synthase to generate ATP the primary energy currency in cells.
Pathways
Both COX4 and COX4L2 integrate into key metabolic pathways such as oxidative phosphorylation and the respiratory chain. Their role in the electron transport chain connects them to other mitochondrial proteins like cytochrome c and ATP synthase. This collaboration is important for the efficient conversion of nutrients into usable cellular energy supporting essential metabolic functions.
Disruptions in the function of COX4 and COX4L2 relate to conditions such as mitochondrial myopathy and Leigh syndrome. Mutations or deficiencies in these proteins can impair electron transport and reduce ATP generation leading to muscle weakness or neurodegenerative symptoms. Cytochrome c and other components of the electron transport chain often exhibit related dysfunctions in these mitochondrial diseases.