Abcam, ab199335, Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LGR5 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3065Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab199335 is the carrier-free version of
ab75850
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LGR5 also known as leucine-rich repeat-containing G-protein coupled receptor 5 is a transmembrane receptor primarily involved in the regulation of stem cell function. With a molecular mass of approximately 99 kDa LGR5 is highly expressed in intestinal crypts hair follicles and other tissues with proliferative capacity. This receptor functions through the activation of downstream signaling pathways upon binding with its ligand R-spondin. Often referred to as GPR49 LGR5 plays an essential role in embryonic development and adult tissue homeostasis.
Biological function summary
LGR5 marks adult stem cells in several rapidly self-renewing tissues. It forms part of a receptor complex that enhances Wnt signaling important for maintaining stemness and controlling cell fate. LGR5's activity ensures the self-renewal and differentiation balance within stem cell niches. The protein acts as a reliable stem cell marker in both intestinal and hair follicle stem cells facilitating their identification and isolation for research or therapeutic purposes.
Pathways
LGR5 interacts significantly with the Wnt signaling pathway playing a critical role in its activation. It associates with the R-spondin and LRP5/6 pathway components to potentiate Wnt signals thereby influencing cellular functions like proliferation and differentiation. LGR5's role in this pathway connects it with other key proteins such as β-catenin and TCF which are important for transcriptional regulation driven by Wnt pathway activation.
LGR5 expression links with colorectal cancer and medulloblastoma. In colorectal cancer overexpression of LGR5 suggests uncontrolled Wnt signaling leading to excessive cell proliferation. This association highlights its potential as a target for cancer therapy. Moreover LGR5 interacts with proteins like APC in tumor suppression and TP53 whose mutations often accompany tumorigenesis. Understanding LGR5's function and regulation helps develop therapeutic strategies for related pathologies.