Abcam, ab200781, Alexa Fluor® 488 Anti-TRIM25/EFP antibody [EPR7315]

Size: 100µL
Rabbit Recombinant Monoclonal TRIM25/EFP antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7315,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle|Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRIM25 also known as EFP is a protein involved in antiviral response mechanisms. TRIM25 has a molecular mass of approximately 70 kDa and is part of the TRIM (Tripartite Motif) family which contains three distinct zinc-binding domains followed by a coiled-coil region. This protein exhibits strong expression in a variety of tissues including lung liver and intestines. As a member of the E3 ubiquitin ligase enzyme family TRIM25 plays a direct role in protein ubiquitination which tags specific proteins for degradation by the proteasome.
Biological function summary
TRIM25 functions as a significant modulator of the innate immune response against viral infections. In cells it acts within the RIG-I signaling complex directly promoting the RIG-I-MAVS pathway that detects viral RNA. TRIM25 mediates the ubiquitination of RIG-I allowing for its proper activation and subsequent signal transduction facilitating a robust antiviral state. TRIM25's role extends beyond antiviral defenses to include regulatory functions in cell proliferation and differentiation.
Pathways
TRIM25 engages actively in the innate immune signaling and JAK-STAT pathways. It interacts and cooperates with RIG-I in the recognition and response to viral tRNA leading to the production of interferons that establish an antiviral environment. Furthermore TRIM25 also connects with proteins like TRIM21 which further engages the immune response. This interaction enhances the overall ability of cells to fend off viral pathogens efficiently.
Altered TRIM25 function is associated with conditions like systemic lupus erythematosus (SLE) and various cancers. In the context of SLE TRIM25's regulatory role in innate immunity can affect the severity and progression of this autoimmune disorder. Additionally in some cancers abnormal TRIM25 expression can influence cell cycle regulation and metastasis. Through its ubiquitination activities TRIM25 interacts directly with proteins such as p53 affecting tumor suppression pathways and contributing to the complexity of disease development and progression.