Abcam, ab201119, Protease and Phosphatase Inhibitor Cocktail

Size: 1Kit
Protease and phosphatase inhibitor cocktail (ab201119) prevents both proteolytic degradation and phosphatase activities during protein extraction and purification from mammalian tissues and cells. - AEBSF free and mass spectroscopy compatible - Contains protease and phosphatase inhibitors at optimized concentrations - Proven performance: cited in over 50 publications
Product details:
To protect against both protease and phosphatase in one solution try our dual cocktail
EDTA-free also available
AEBSF free and mass spectroscopy compatible – water soluble.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases
Target phosphatases - serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases
Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate, EDTA disodium salt.
Supplied as 1 vial of cocktail in water (1 ml) and 1 vial of EDTA disodium salt (0.5 mM in 1 ml of water)
Preparation:
The recommended starting dilution is 1:9
Visit our
inhibitor cocktails page
for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
This product is manufactured by BioVision, an Abcam company and was previously called K283 EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail. K283-1 is the same size as the 1 kit size of ab201119.
Individual components and concentration:
Aprotinin (bovine lung):
serine protease inhibitor (0.08 mM)
Bestatin:
aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate:
serine/cysteine protease inhibitor (2 mM)
β-Glycerophosphate:
serine/threonine phosphatase inhibitor (10 mM)
Sodium fluoride:
acid phosphatase and serine/threonine phosphatase inhibitor (200 mM)
Sodium orthovanadate:
protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
Sodium pyrophosphate decahydrate:
serine/threonine phosphatase inhibitor (10 mM)
EDTA disodium salt:
metalloprotease inhibitor (0.5 mM)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C