Abcam, ab202406, Mouse CCL27 ELISA Kit (CTACK)

Size: 1 x 96Tests
Mouse CCL27 ELISA Kit (CTACK) is a single-wash 90-min Simplestep used to quantify Mouse CCL27 (CTACK) with a sensitivity of 9.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 9.3 pg/mL,
Range:23.4 - 1500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse CCL27 ELISA Kit (CTACK) (ab202406) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL27 (CTACK) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CCL27 (CTACK) with 9.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CCL27 is a chemotactic factor that attracts skin-associated memory T-lymphocytes, and binds to CCR10. It may play a role in cell migration during embryogenesis, and may mediate lymphocyte homing to cutaneous sites. Nuclear forms of CCL27 may facilitate cellular migration by inducing cytoskeletal relaxation. There is evidence that CCL27 is strongly upregulated in atopic dermatitis, contact dermatitis, and psoriasis skin lesions.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.
Biological function summary
CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.
Pathways
The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.
CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.