Abcam, ab202800, Recombinant human MDC protein

Size: 5µg
Recombinant human MDC protein is a Human Full Length protein, in the 25 to 93 aa range, expressed in Escherichia coli, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.
Key facts
Purity:>97% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGE, FuncS, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Fully biologically active when compared to standard. Determined by its ability to chemoattract human T cells using a concentration range of 10.0-100.0 ng/ml.,
Accession:O00626,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in water,
Storage buffer:pH: 7.4Constituents: 97% Phosphate Buffer, 2.9% Sodium chloride

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.
Biological function summary
MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.
Pathways
MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.
MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.