Abcam, ab203223, Anti-ompF antibody

Size: 100µL
Rabbit Polyclonal ompF antibody. Suitable for WB, IHC-P and reacts with Bacteria samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Bacteria,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ompF protein known as Outer membrane protein F serves as a porin in the outer membrane of Escherichia coli. It facilitates the passive diffusion of small molecules across the cell membrane. This protein has a trimeric structure where each monomer forms a beta-barrel. Each of the monomers has a mass of about 38 kDa. The ompF protein is typically expressed in response to varying environmental factors like osmolarity and is important for regulating molecule entry into the bacterial cell.
Biological function summary
OmpF plays a significant role in maintaining homeostasis and nutrient uptake in bacteria. It forms part of the beta-barrel assembly machinery complex which handles the insertion and folding of beta-barrel proteins into the outer membrane. This protein adjusts bacteria's permeability properties ensuring necessary ions and small nutrients reach the cell’s interior while controlling the access of harmful substances.
Pathways
OmpF participates in nutrient uptake and ion transport pathways significantly affecting bacterial survival and adaptation processes. OmpF interacts with other proteins in the porin family such as ompC which collectively regulate ion passage and osmotic balance within the bacterial cell envelope. These actions occur in pathways related to membrane permeability which are vital for bacteria responding to environmental stress.
OmpF has a linkage to antibiotic resistance mechanisms in pathogenic bacteria. Alterations in the ompF protein or expression can reduce antibiotic susceptibility complicating treatment of infections caused by Escherichia coli. This protein has connections with various resistance proteins where modulating such pathways may enhance antibiotic effectiveness. Understanding its role and interactions can guide strategies to counteract antimicrobial resistance in clinical settings.