Abcam, ab203394, Anti-Semaphorin 3F antibody

Size: 100µL
Rabbit Polyclonal Semaphorin 3F antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SEMA3F aa 700 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SEMA3F aa 700 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q13275

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Semaphorin 3F also known as Sema3F is a secreted protein that functions as a guidance cue in various cellular processes. It has a molecular mass of approximately 88 kDa. Sema3F is a part of the class 3 semaphorins and is characterized by its sema domain PSI domain and Ig-like C2-type domain. It is expressed widely across multiple tissues including the nervous system lungs and heart. These expression patterns suggest a diverse role in both embryonic development and adult tissues.
Biological function summary
Semaphorin 3F plays an important role in axon guidance cell migration and angiogenesis. It mediates these effects through its interaction with neuropilin-2 and plexins forming functional receptor complexes on target cells. Particularly in the nervous system it repels axons helping to shape neural circuits. In the context of angiogenesis Sema3F limits new blood vessel formation by inhibiting endothelial cell movement thereby impacting vascular development.
Pathways
Semaphorin 3F engages in the axon guidance and VEGF signaling pathways. Through its participation in the axon guidance pathway it influences the migration and directionality of neuronal axons. Additionally in the VEGF pathway Sema3F interacts with proteins like VEGFR and neuropilins serving as an inhibitory element that counterbalances pro-angiogenic signals. These interactions help maintain the balance between promoting and inhibiting vessel formation.
The dysregulation or altered function of Semaphorin 3F has significant implications in cancer progression and neurological disorders. Reduced expression of Sema3F correlates with increased tumor growth and metastasis in cancers such as glioblastoma and lung cancer. In neurological disorders like epilepsy decreased Sema3F levels contribute to aberrant neural connectivity. Its interaction with related proteins such as neuropilin-2 and plexins highlights its clinical relevance and potential as a therapeutic target.