Abcam, ab203491, Anti-PDGFR alpha antibody [EPR22059-270]

Size: 20µL / 100µL / 1mL
Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) is a rabbit monoclonal antibody detecting PDGFR alpha in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22059-270,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IP, WB, ICC/IF, I-ELISA, Flow Cyt, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:PDGFR alpha is membrane protein, so enrichment of membrane could help increasing the detection level of PDGFR alpha.

Product details:
Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, I-ELISA, ICC/IF, IHC-Fr, IHC-P and IP.
Abcam's high quality manufacturing and validation processes ensure Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) has been confirmed by Western Blot testing in PDGFR alpha knockout SH-SY5Y cells (
ab275335
Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) has 4 independent reviews from customers.
Anti-PDGFR alpha antibody [EPR22059-270] (ab203491) specifically detects PDGFR alpha (UniProt ID: P16234; Molecular weight: 121kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR22059-270 -
ab234965
Antibody clone EPR22059-270 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, PE, Alexa Fluor
ab270085
ab270086
ab307855
The platelet-derived growth factor receptor alpha (PDGFRA) is a cell surface tyrosine kinase receptor that plays a significant role in tumor growth, invasion and angiogenesis in oncology often serving as a therapeutic target due to its overexpression in various cancers, particularly in gastrointestinal stromal tumors (GISTs),
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.
Biological function summary
PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.
Pathways
PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.
PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.