Abcam, ab203569, Anti-AF6 antibody [A7L9H48]

Size: 100µg
Rabbit Recombinant Monoclonal I Afadin antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Afdn aa 1800 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:A7L9H48,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Afdn aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9QZQ1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AF6 also known as Afadin or anti-i is a protein involved in cell signaling and adhesion mechanisms. It weighs approximately 180 kDa and plays a role in the formation of tight junctions influencing cellular architecture and communication. AF6 is expressed in a variety of tissues including epithelial cells indicating its involvement in maintaining tissue integrity. The protein localizes at cellular junctions where it interacts with other junctional components facilitating connections between adjacent cells.
Biological function summary
AF6 contributes to cell-to-cell contact by organizing cytoskeletal elements and signaling elements. It exists within the nectin-afadin complex which is critical for the regulation of tight junctions and adherens junctions. This positioning allows AF6 to modulate processes like cell migration and tissue morphogenesis. Through its interactions with actin filaments and other junctional proteins AF6 has a significant role in maintaining proper cellular organization and stability.
Pathways
AF6 participates actively in the Ras signaling and Rap1 pathways. Within the Ras pathway AF6 associates with proteins like Ras and Rap1 which regulate various cellular outcomes including proliferation and differentiation. AF6's involvement with these pathways enhances its role in influencing cell dynamics and signaling decisions. AF6's function in these signaling pathways underlines its participation in normal cell function and development.
AF6 has links to cancer progression and neurological disorders such as autism. The protein’s interaction with Ras influences oncogenic signaling which can lead to aberrant cell growth and metastasis in cancers. Additionally disruptions in AF6 function or expression levels have associations with autism spectrum disorders as AF6 affects neural cell adhesion and signaling pathways. The protein’s interactions with junctional proteins like cadherins and nectins in diseased states suggest its potential as a therapeutic target.