Abcam, ab204131, Anti-PFKM + PFKP antibody [EPR17314]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PFKP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 21 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17314,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PFKM and PFKP are key enzymes involved in glycolysis. These targets are commonly referred to as phosphofructokinase muscle (PFKM) and phosphofructokinase platelet (PFKP). PFKM has a molecular mass of approximately 85 kDa while PFKP is slightly larger at around 84 kDa. PFKM is expressed mainly in muscle tissues whereas PFKP is found in platelets and other tissues like the brain. These enzymes catalyze the conversion of fructose-6-phosphate to fructose-16-bisphosphate using ATP an important regulatory step in glycolysis.
Biological function summary
PFKM and PFKP play significant roles in energy metabolism. They are part of the phosphofructokinase enzyme complex which regulates glycolytic flux. PFKM is important for muscle energy production during high-intensity exercises where anaerobic respiration predominates. PFKP on the other hand manages glycolytic rates in tissues like brain and platelets which have unique energy demands. The activity of these enzymes is controlled by various metabolites reflecting cellular energy status.
Pathways
PFKM and PFKP are central components of the glycolysis pathway a critical energy-yielding process. Glycolysis not only provides ATP but also intermediates for other pathways like the Krebs cycle. Both PFKM and PFKP interact with enzymes like aldolase and pyruvate kinase coordinating energy production. Their regulation ensures proper adaptation to varying energy requirements across different cell types and tissues.
PFKM mutations associate with glycogen storage disease type VII (Tarui's disease) where affected individuals suffer from exercise intolerance and muscle cramps. This connection highlights the importance of PFKM in muscle energy production. PFKP has been linked to cancer metabolism as it is involved in adapting glycolysis rates in tumors often called the Warburg effect. Dysregulation of these enzymes can alter metabolic processes interacting with proteins like lactate dehydrogenase contributing to disease progression.