Abcam, ab204252, Alexa Fluor® 488 Anti-AKT3 + AKT2 + AKT1 antibody [Y89]

Size: 100µL
Rabbit Recombinant Monoclonal AKT1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y89,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein kinase AKT also known as protein kinase B (PKB) includes three isoforms: AKT1 AKT2 and AKT3. These play a central role in cellular processes. AKT1 has a molecular weight of approximately 56 kDa AKT2 has around 55 kDa and AKT3 similarly weighs about 54 kDa. These isoforms of AKT are expressed in various tissues but AKT1 is abundantly found in most tissues AKT2 is often present in insulin-responsive tissues like skeletal muscle and adipose tissue and AKT3 is mainly in the brain. The activation of AKT involves phosphorylation which increases their kinase activity significantly. The isoforms have unique and overlapping functions offering distinct mechanical roles in cellular signaling.
Biological function summary
The AKT kinase family plays a major role in regulating vital cellular functions including metabolism cell proliferation survival and growth. It does so by catalyzing phosphorylation of a range of substrates within these pathways. AKT is often involved in complexes with other proteins to achieve these biological effects. It impacts the regulation of glycogen synthase kinase 3 (GSK3) involved in insulin response and mTOR which controls cell growth. These wide-ranging functions make AKT proteins essential in maintaining cellular homeostasis.
Pathways
AKT proteins are important components of the PI3K/AKT/mTOR signaling pathway and the insulin signaling pathway. Within these pathways they interact with and modulate activities of related proteins such as PI3K PDK1 and mTOR. This regulation helps control the balance between anabolic and catabolic processes influencing cell survival growth and metabolism. The importance of AKT kinases in these pathways marks them as key regulators of cellular responses to external and internal stimuli.
AKT isoforms have strong connections to cancer and diabetes. Over-activation of AKT pathways frequently occurs in cancerous cells contributing to uncontrolled cell growth and survival. In diabetes AKT2 is particularly implicated due to its role in insulin signaling influencing how cells process glucose. Dysregulation of AKT pathways has been linked to the development and progression of these diseases where proteins like mTOR in cancer and IRS1 in diabetes show direct relation to AKT function and malfunctions.