Abcam, ab204308, Anti-Nesprin 2 antibody

Size: 100µL
Rabbit Polyclonal Nesprin 2 antibody. Suitable for ICC, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SYNE2 aa 4200-4350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SYNE2 aa 4200-4350. The exact immunogen used to generate this antibody is proprietary information.Q8WXH0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nesprin-2 also known as Nesprin-2 or ABP-Rince2002 is a mechanical protein that plays an important role in the structure of cells. This protein is part of the spectrin repeat-containing nuclear envelope (KASH) proteins with a large mass. It is mainly expressed in tissues such as skeletal muscle the heart and brain. Nesprin-2 is found in the outer nuclear membrane where it functions as a mediator linking the nuclear envelope to the cytoskeleton facilitating mechanical coupling between the nucleus and the cytoplasm.
Biological function summary
Nesprin-2 is involved in various cellular processes that contribute to nuclear positioning and cell movement. It interacts with other nuclear envelope proteins like SUN proteins forming the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex which ensures mechanical communication within cells. This complex supports the anchoring and movement of the nucleus during cytoskeletal changes. Nesprin-2's anchoring abilities make it essential for maintaining cellular integrity and adaptability during mechanical stress.
Pathways
Nesprin-2 participates in mechanotransduction pathways that convert mechanical stimuli into biochemical signals. It plays a critical role in the actin cytoskeleton organization and nuclear positioning pathways. Through these pathways Nesprin-2 interacts with proteins such as F-actin which are important for maintaining cell shape and mobility. The interactions between Nesprin-2 and cytoskeletal elements help facilitate cell signaling in response to mechanical forces.
Nesprin-2 is linked to muscular dystrophies and cardiomyopathies. In muscular dystrophies mutations in Nesprin-2 or its interacting partners like Emerin impair nuclear-cytoskeletal coupling leading to muscle weakness. In cardiomyopathies Nesprin-2 dysfunction affects cardiac muscle integrity and function where it may interact with proteins like Lamin A/C. Understanding these connections offers potential avenues for researching therapeutic interventions for these conditions.