Abcam, ab204515, Anti-Cdc34 antibody [EPR16808]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Cdc34 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16808,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Cdc34 protein also known as Ubc3 is a ubiquitin-conjugating enzyme with a molecular mass of about 35 kDa. It is expressed in various tissues with high presence in actively dividing cells. Cdc34 plays a significant role in cell cycle regulation particularly during the transition from G1 to the S phase. It facilitates the attachment of ubiquitin molecules to target proteins marking them for degradation by the proteasome which is important for controlling the abundance of specific proteins involved in cell cycle progression.
Biological function summary
Cdc34 functions as a member of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex. This complex targets a range of key regulatory proteins for ubiquitination influencing processes such as DNA replication and repair. By mediating protein degradation Cdc34 helps regulate the cellular levels of cyclins which are essential for proper cell cycle advancement. Its activity ensures timely degradation of these cyclins thereby preventing unscheduled cell cycle transitions that can lead to cellular dysfunction.
Pathways
Cdc34 is integrated in the ubiquitin-proteasome pathway and the cell cycle pathway. In these contexts Cdc34's activity closely interacts with proteins like Skp2 another component of the SCF complex which regulates G1-S phase transition. Cdc34-related pathways ensure that protein turnover is tightly controlled which is essential for maintaining cellular homeostasis and responding to internal and external signals during cell proliferation.
Cdc34 links to cancer and neurodegenerative disorders. Alterations in Cdc34 function can result in the aberrant degradation of regulatory proteins contributing to unchecked cell proliferation a hallmark of cancer. Furthermore imbalances in ubiquitin-proteasome pathway components including Cdc34 relate to neurodegenerative diseases where protein aggregation occurs. Connections to proteins such as p27 which regulates cell cycle and accumulation issues in neurological tissues highlight the significance of Cdc34 in disease mechanisms.