Abcam, ab204604, Anti-Ankyrin repeat domain-containing protein 65 antibody

Size: 100µL
Rabbit Polyclonal Ankyrin repeat domain-containing protein 65 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANKRD65 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ANKRD65 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.E5RJM6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ankyrin repeat domain-containing protein 65 also known as RFXANK is a protein with a molecular mass of approximately 31 kDa. This protein contains ankyrin repeats which are known for mediating protein-protein interactions. Ankyrin repeat domain-containing protein 65 is localized mainly in the cytoplasm and nucleus of cells. It expresses in various tissues including those involved in immune system like T-cells and dendritic cells highlighting its potential roles in cellular processes in these areas.
Biological function summary
Ankyrin repeat domain-containing protein 65 functions in transcription regulation. It forms part of the heterotrimeric RFX complex which is vital for the transcription of MHC class II genes. The protein's ankyrin repeats facilitate the interactions necessary for the assembly of this complex and ensure proper binding to the MHC promoters. Through these molecular actions ankyrin repeat domain-containing protein 65 influences immune responses showcasing its role in adaptive immunity.
Pathways
Ankyrin repeat domain-containing protein 65 is heavily involved in the MHC class II pathway. Its role within the RFX complex affects the expression of MHC class II molecules important for antigen presentation to CD4+ T cells. This protein therefore connects with other proteins in these pathways such as CIITA RFX5 and RFXAP to modulate gene expression and maintain immune function integrity.
Defects in ankyrin repeat domain-containing protein 65 have associations with bare lymphocyte syndrome type II a rare immunodeficiency disorder. This disorder stems from defective MHC class II expression linking the protein to important immune functions. Additionally altered ankyrin repeat domain-containing protein 65 activity may relate to autoimmunity given its role in immune regulation. Through these disease processes the protein interacts with CIITA and other MHC class II complex proteins underlining its significance in maintaining immune homeostasis and preventing immune-related pathologies.