Abcam, ab204713, Phosphoenolpyruvic acid (PEP) Assay Kit

Size: 100Test
Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713) provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples.
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Tissue Lysate, Urine, Plasma, Serum, Other biological fluids, Cell Lysate,
Assay type:Enzyme activity,
Sensitivity:= 1 µM,
Assay Platform:Microplate reader

Product details:
Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713) provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K365 PEP Colorimetric/Fluorometric Assay Kit. K365-100 is the same size as the 100 test size of ab204713.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphoenolpyruvate often abbreviated as PEP is an important metabolic intermediate in glycolysis. Alternate names include phosphoenolpyruvic acid. It is a phosphorylated compound with a molar mass of 167.07 g/mol. In cells PEP is generated via the enolase enzyme converting 2-phosphoglycerate to PEP. Its high-energy phosphate group makes it critical for ATP generation in glycolysis. This molecule is expressed in the cytoplasm of all living organisms reflecting a fundamental role in cellular metabolism.
Biological function summary
The role of phosphoenolpyruvate extends beyond glycolysis. It acts as a significant player in gluconeogenesis aiding in the conversion of lactate and other substrates into glucose. PEP is an important precursor for several biosynthetic pathways including amino acid synthesis. It is not part of a molecular complex but interacts with several enzymes such as pyruvate kinase that facilitate its conversion into pyruvate releasing energy stored in its phosphate bond.
Pathways
Phosphoenolpyruvate is central to both glycolysis and gluconeogenesis. It stands at a critical juncture in the glycolytic pathway where the PEP to pyruvate conversion catalyzed by pyruvate kinase is a major step for ATP synthesis. In gluconeogenesis PEP converts back to glucose ensuring energy balance during fasting states. PEP is closely related to proteins such as pyruvate kinase and enolase which are key players in the glycolytic and gluconeogenic pathways respectively.
Alterations in phosphoenolpyruvate metabolism connect to various health conditions including cancer and diabetes. For instance dysregulated glycolysis involving PEP often occurs in cancer cells exhibiting the Warburg effect characterized by high rates of glycolysis even under oxygenated conditions. Diabetes also shows links to abnormal PEP levels affecting glucose production and metabolism. Pyruvate kinase related to PEP is another protein implicated in these disorders highlighting its role in metabolic and energy homeostasis.