Abcam, ab205074, Mouse Fetuin A ELISA Kit

Size: 1 x 96Tests
Mouse Fetuin A ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Fetuin A in Mouse in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.846 ng/mL,
Range:3.125 - 100 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Fetuin A in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A protein in mouse plasma, serum and urine.
In this assay the Fetuin present in samples reacts with the anti-Fetuin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Fetuin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Fetuin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Fetuin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Fetuin in the test sample. The quantity of Fetuin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C