Abcam, ab205270, Anti-active YAP1 antibody [EPR19812]

Size: 20µL / 100µL / 1mL
Anti-active YAP1 antibody [EPR19812] (ab205270) is a rabbit monoclonal antibody detecting active YAP1 in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 90 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19812,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab205270 is specific to the active (non-phosphorylated) form of YAP1.

Product details:
Anti-active YAP1 antibody [EPR19812] (ab205270) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P and WB.
Abcam's high quality manufacturing and validation processes ensure Anti-active YAP1 antibody [EPR19812] (ab205270) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-active YAP1 antibody [EPR19812] (ab205270) has been confirmed by Western Blot testing in active YAP1 knockout HAP1 cells.
Anti-active YAP1 antibody [EPR19812] (ab205270) has 25 independent reviews from customers.
Anti-active YAP1 antibody [EPR19812] (ab205270) specifically detects active YAP1 (UniProt ID: P46937; Molecular weight: 55kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR19812 -
ab223126
Antibody clone EPR19812 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, Alexa Fluor
488, Alexa Fluor
594, Alexa Fluor
568, Alexa Fluor
555, Alexa Fluor
ab225440
ab225441
ab311764
ab313045
ab313246
ab321359
YAP1 is crucial in disease as it regulates cell proliferation, differentiation and survival, playing significant roles in cancer, liver diseases and orthopaedic degenerative conditions.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Active YAP1 also known as Yes-associated protein 1 is a transcription co-activator with a mass of approximately 65 kDa. It plays a mechanical role in the regulation of gene expression by interacting with transcription factors and enhancing their ability to drive transcription. YAP1 is expressed in various tissues including the liver intestine and neural tissues. Also found in the cytoplasm and nucleus its expression is tightly regulated because of its involvement in maintaining cell proliferation and survival.
Biological function summary
Active YAP1 functions as an important regulator in the Hippo signaling pathway. It acts as an effector that transmits signals from upstream kinases to control organ size by modulating cell growth and apoptosis. YAP1 associates with the TEAD transcription factor family to regulate the expression of genes involved in cell proliferation and differentiation. The complex formed between YAP1 and TEAD is essential for its biological activity influencing processes like stem cell renewal and tissue regeneration.
Pathways
Active YAP1 has a central role in the Hippo signaling pathway important for controlling cellular behaviors such as growth and survival. In this pathway YAP1 interacts with other proteins like LATS1/2 kinases to control its activity through phosphorylation and subcellular localization. Akt/mTOR pathway also connects with YAP1 integrating signals that affect cell growth and metabolism. These interactions highlight YAP1’s position as a pivotal node in maintaining cellular homeostasis.
Active YAP1 is implicated in several conditions including cancer and liver disorders. Overexpression or dysregulation of YAP1 leads to oncogenic transformation closely associating it with cancers such as hepatocellular carcinoma and breast cancer. In liver disorders YAP1's activity affects fibrosis and regeneration by interacting with proteins like β-catenin modulating pathways linked to tissue repair and scarring. Understanding YAP1’s role offers potential therapeutic targets for managing these diseases.