Abcam, ab205924, Anti-IL-1 beta antibody

Size: 100µg
Rabbit Polyclonal IL-1 beta antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 50 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Il1b.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Mouse Il1b.P10749,
Specificity:ab205924 is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL1 alpha based on a neutralization assay. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL-1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.

Properties and Storage Information:
Form-Liquid, Purification technique-Ion exchange chromatography DEAE-C, Purification notes-This is an IgG preparation of whole rabbit serum purified by DEAE fractionation., Storage buffer-Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle