Abcam, ab206987, Mouse beta IG H3 ELISA Kit

Size: 1 x 96Tests
Mouse beta IG H3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse beta IG H3 with a sensitivity of 40 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 40 pg/mL,
Range:0.16 - 10 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse beta IG H3 ELISA Kit (ab206987) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of beta IG H3 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse beta IG H3 with 40 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse beta IG H3 (TGFBI) is a 683 amino acid (aa) protein with a 23 aa signal peptide, one EMI domain, four FAS1 domains, and one RGD motif. Mouse beta IG H3 is expressed as a 75 kDa protein with no post-translational additions. Following secretion, cleavages at multiple positions near the C-terminal end release peptides with pro-apoptotic activity. The four FAS1 domains of Mouse beta IG H3 contain YH motifs, which allow Mouse beta IG H3 to bind to matrix fibronectin, collagen I, collagen VI, biglycan, and decorin. Mouse beta IG H3 is an adaptor protein that is induced in most cell types in response to TGF-beta stimulation. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts. Mouse beta IG H3 shares 91% aa sequence identity with human and porcine beta IG H3.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C