Abcam, ab207000, Thrombin cleavage kit

Size: 500Test
Thrombin cleavage kit (ab207000) efficiently removes tags (e.g. 6x His tag) from recombinant fusion proteins containing an accessible thrombin cleavage sequence. - Suitable for recombinant protein purification and tag removal - Contains sufficient active thrombin enzyme to cleave up to 5 mg of the target protein - Kit includesThrombin cleavage control protein
Key facts
Sample types:Purified protein

Product details:
Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.
Thrombin is a valuable biochemical tool due to its high proteolytic specificity. A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with thrombin, affinity tags or fused proteins can be separated from the target protein.
The ab207000 kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing a thrombin-specific cleavage site.
Overview
The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein.
A 6x His-tagged protein containing the thrombin cleavage site is included as a cleavage control protein.
Following cleavage of the target protein, thrombin can be removed by passing the reaction mix through a Heparin Sepharose
column.
This product is manufactured by BioVision, an Abcam company and was previously called K377 Thrombin Cleavage Kit. K377-500 is the same size as the 500 test size of ab207000.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C