Abcam, ab207924, Pig Lipocalin-2 ELISA Kit

Size: 1 x 96Tests
Pig Lipocalin-2 ELISA Kit is a sandwich ELISA designed to quantify Pig Lipocalin-2 with a sensitivity of 1 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 4 - 400 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum,
Reacts with:Pig,
Assay type:Sandwich (quantitative),
Sensitivity:= 1 pg/mL,
Range:4 - 400 pg/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Pig Lipocalin-2 ELISA Kit ab207924 is a sandwich ELISA to measure Pig Lipocalin-2 in serum, plasma, urine with a sensitivity of 1 pg/ml.
How the assay works
The assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against pig NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Pig Lipocalin-2 ELISA Kit ab207924 protocol summary
1. Add standards, samples and any controls to appropriate wells
2. Wash
Add Biotinylated monoclonal detection antibody and incubate
4. Wash.
5. Add HRP-conjugated streptavidin.
5. Wash
6. Add TMB
7. Add Stop Solution and read at 450 nm
NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are small secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL's ability to bind bacterial siderophores. As NGAL was first reported as oncogene 24p3 in the mouse, this name is still occasionally used to identify NGAL in other species.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C