Abcam, ab208342, Mouse DLL1 ELISA Kit

Size: 1 x 96Tests
Mouse DLL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse DLL1 with a sensitivity of 24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 24 pg/mL,
Range:62.5 - 4000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse DLL1 ELISA Kit (ab208342) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DLL1 protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse DLL1 with 24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
DLL1 (Delta-like protein 1) is a transmembrane glycoprotein that is needed during development for cell-to-cell communication in embryos and in the nervous system development. In adult tissues, DLL1 is involved in morphogenesis and remodeling and is overexpressed in several types of cancers.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein known as Delta-like 1 (DLL1) also referred to as DLL 1 or DLL1 protein is an essential component in various cellular mechanisms. DLL1 typically has a molecular mass of approximately 80 kDa. This protein is mainly expressed in tissues related to development such as the nervous system bone marrow and certain parts of the embryonic organs. DLL1 functions mechanic-wise as a ligand for the Notch receptor family playing a role in signal transduction and influencing cell fate decisions.
Biological function summary
Delta-like 1 protein is involved in several cell differentiation processes impacting tissue development. The protein is a member of the Notch signaling pathway which is important for mediating communication between adjacent cells. It interacts with Notch receptors to regulate transcriptional activities and promote different cell lineages. DLL1 does not function in isolation; it works as a part of a larger protein complex that includes intracellular domains and other signal modifiers.
Pathways
Delta-like 1 fits into the Notch signaling pathway and the Wnt pathway. In the Notch signaling pathway DLL1 engages with Notch receptors to drive transcriptional responses important for embryogenesis and cell fate choices. In the Wnt pathway DLL1 interacts to modulate cellular growth and differentiation often in conjunction with proteins such as Jagged1 and Notch1. These pathways are essential in maintaining stem cell niches and influencing neurogenesis.
Delta-like 1 protein bears significance in oncological and congenital disorders. Dysregulation of DLL1 is associated with cancers like T-cell acute lymphoblastic leukemia where altered Notch signaling plays a role in tumor progression. Additionally abnormalities in DLL1 expression relate to congenital heart defects indicating its role in cardiac development. DLL1's interaction with Notch receptors such as Notch3 appears significant in these contexts making it a potential target for therapeutic intervention in both cancer and congenital disorder management.