Abcam, ab208942, Anti-c-Fos antibody [2H2]

Size: 100µL
Anti-c-Fos antibody [2H2] (ab208942) is a mouse monoclonal antibody detecting c-Fos in Western Blot, IHC-P, IHC-FrFl, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 100 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2H2,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WB, ICC/IF, IHC-P, IHC-FrFlSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human FOS.P01100,
Specificity:High levels of background are often observed when staining mouse tissue with mouse antibody. For mouse samples use ab222699.

Product details:
Anti-c-Fos antibody [2H2] (ab208942) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-FrFl, IHC-P and WB.
Anti-c-Fos antibody [2H2] (ab208942) was first used in a scientific publication in 2017 and has been cited over 100 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-c-Fos antibody [2H2] (ab208942) has high sensitivity and specificity.
The specificity of Anti-c-Fos antibody [2H2] (ab208942) has been confirmed by WB testing in c-Fos knockout PC-12 cells (ab281615).
Anti-c-Fos antibody [2H2] (ab208942) has 12 independent reviews from customers.
Anti-c-Fos antibody [2H2] (ab208942) specifically detects c-Fos (UniProt ID: P01100; Molecular weight: 41kDa) and is sold in 100 µL selling sizes.
c-Fos is an immediate-early gene widely used in neuro research for its role as a marker of neuronal activation. It is rapidly expressed in response to various stimuli, making it an excellent indicator of neuronal activity and functional mapping. Researchers use c-Fos to study brain activity patterns, synaptic plasticity and the effects of drugs or other interventions on the central nervous system, making it essential for understanding dynamic changes in brain function.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Affinity purified from tissue culture supernatant., Storage buffer-Preservative: 0.03% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.
The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
Pathways
C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.
Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.