Abcam, ab209692, Anti-TPP1 antibody [EPR16537] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TPP1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16537,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.

Product details:
ab209692 is the carrier-free version of
ab195234
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tripeptidyl peptidase I (TPP1) also known as CLN2 is a lysosomal serine protease with a molecular mass of about 61 kDa. TPP1 is expressed in numerous tissues with notable activity in the brain and retinal cells. Mechanically TPP1's primary function includes cleaving N-terminal tripeptides from substrates an important step in protein degradation and turnover within the lysosome.
Biological function summary
This protease plays an essential role in maintaining cellular function by preventing the accumulation of partially degraded proteins. TPP1 exists as part of a lysosomal enzyme complex involved in the catabolic process that recycles cellular components. Its action ensures proper protein homeostasis protecting cells from toxic buildup that can lead to cellular dysfunction.
Pathways
TPP1 is a part of the lysosomal degradation pathway critical for cellular waste management and nutrient recycling. This pathway intersects with broader metabolic routes where TPP1 activity supports the catabolism of intracytoplasmic substrates. TPP1 also interacts with proteins such as cathepsin D another enzyme contributing to proteolytic pathways to maintain the balance and efficiency of protein degradation processes.
TPP1 mutations are associated with neuronal ceroid lipofuscinosis type 2 (CLN2 disease) a rare neurodegenerative disorder. This disease involves progressive brain and retinal degeneration due to lysosomal storage malfunction. The TPP1 connection to CLN2 disease highlights its essential role in neurological health. Additionally TPP1 has links to Batten disease where compromised enzyme function leads to severe neurological symptoms. Other proteins like CLN3 may also interact with TPP1 in these disease contexts influencing their progression and pathology.