Abcam, ab210237, PerCP/Cy5.5® Anti-CD45.1 antibody [A20]

Size: 100µg
Mouse Monoclonal CD45 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:A20,
Isotype:IgG2a,
Light chain type:kappa,
Conjugation:PerCP/Cy5.5®,
Excitation/Emission:Ex: 482nm, Em: 695nm,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody reacts with mouse CD45.1, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). This antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from tissue culture supernatant and conjugated under optimal conditions, with unreacted dye removed from the preparation., Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD45.1 also known as Leukocyte Common Antigen (LCA) or Protein Tyrosine Phosphatase Receptor Type C (PTPRC) is a protein expressed on the surface of all nucleated hematopoietic cells. It has a molecular mass of approximately 180-220 kDa. This protein acts as a receptor-type protein tyrosine phosphatase. CD45.1 is an essential marker in the immune system particularly found on T cells B cells and other white blood cells. The expression of CD45.1 is seen across leukocytes where it plays a significant role in cell signaling and antigen receptor signaling.
Biological function summary
CD45.1 influences the immune response by modulating signals through antigen receptors. It operates as a single-pass type I membrane protein and is not known to be part of a larger complex. This protein is important in the activation and regulation of T-cells and B-cells by fine-tuning the threshold of signals required for cell activation. The presence of CD45.1 affects multiple processes in adaptive immunity impacting lymphocyte development and their further activation in immune responses.
Pathways
CD45.1 is integral in lymphocyte activation pathways. It influences the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways by dephosphorylating specific kinases like Lck and Fyn which are important in initiating TCR and BCR signaling cascades. Moreover CD45.1 interacts with other proteins such as CD3 and CD19 being essential in the regulation of immune response and maintaining proper signaling amplitudes to ensure effective immune function.
CD45.1 is intricately linked to autoimmune diseases and leukemia. The protein's dysregulation can lead to improper immune signaling contributing to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In leukemia the altered expression of CD45.1 and related proteins such as CD20 often correlates with disease progression and treatment response. Understanding the roles of CD45.1 can facilitate the development of targeted therapies in these contexts.