Abcam, ab210961, Anti-ACAA1/Beta-ketothiolase antibody [CL2650]

Size: 100µL
Mouse Monoclonal ACAA1/Beta-ketothiolase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACAA1 aa 50-200.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL2650,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ACAA1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.P09110,
Epitope:Binds to an epitope located within the peptide sequence GAIMARIAQFLSDIP as determined by overlapping synthetic peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ACAA1 also known as Beta-ketothiolase is an enzyme involved in key metabolic processes. This enzyme with a molecular mass of approximately 41 kDa plays a pivotal role in the thiolytic cleavage of 3-ketoacyl-CoA an essential step in both fatty acid beta-oxidation and ketogenesis. ACAA1 is expressed in various tissues notably liver and muscle where high rates of fatty acid metabolism occur. As part of the peroxisome it functions effectively to maintain cellular lipid homeostasis.
Biological function summary
ACAA1 facilitates the metabolic breakdown of fatty acids by participating in the final steps of beta-oxidation. It does not form part of a larger enzymatic complex but it operates alongside enzymes like acyl-CoA oxidase and enoyl-CoA hydratase that contribute to the fatty acid oxidation pathway. Through its enzymatic activity ACAA1 directly influences energy production and metabolic rate which are important for maintaining cellular energy balance.
Pathways
Beta-ketothiolase plays an integral role in the fatty acid beta-oxidation and ketogenesis pathways. These pathways are critical for the breakdown of fatty acids into acetyl-CoA which enters the citric acid cycle for energy production. ACAA1 works closely with enzymes such as acetoacetyl-CoA thiolase and HMG-CoA lyase in these processes highlighting its involvement in converting fatty acids into usable energy forms.
Malfunction or deficiency in ACAA1 can lead to issues like ketoacidosis and metabolic dysregulation disorders. The enzyme's dysfunction can result in improper fatty acid metabolism and accumulation of toxic intermediates. ACAA1 shares a connection with HMG-CoA synthase in ketogenesis and any disruption in these pathways may contribute to metabolic diseases impacting energy homeostasis and leading to serious health conditions.