Abcam, ab211154, DNA Damage Assay Kit (AP sites, Colorimetric)

Size: 50Test
DNA damage Assay Kit (AP sites, Colorimetric) ab211154 provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions. - High sensitivity: detects as low as 4-40 AP sites in 10 5 bp DNA - Suitable for use with cells or tissues - Oxidized and reduced DNA standards included for quantitation - 450nm readout - works on any standard microplate reader
Key facts
Detection method:Colorimetric,
Sample types:DNA,
Assay type:Quantitative,
Assay Platform:Microplate reader

Product details:
DNA damage – AP sites – Assay Kit (Colorimetric) (ab211154) is a sensitive assay for the detection of AP sites with a sensitivity of 4-40 AP sites per 10^5 bp.
How the assay works
This DNA damage assay uses an APR (Aldehyde Reactive Probe) that reacts specifically with an aldehyde group on the open ring form of AP sites. AP sites are then tagged with biotin residues that can later be quantified using an streptavidin-enzyme conjugate that is easily detected by absorbance at OD450 nm. The kit has a detection sensitivity range of 4-40 AP sites per 1 x 10^5 bp.
DNA damage assay protocol summary
- ARP-DNA Standard preparation
- DNA purification
- ARP reaction
- Incubation with DNA Binding Solution
- Incubation with Streptavidin-Enzyme conjugate
- Incubation with Substrate Solution
- Measure absorbance at OD 450 nm
How other researchers are using DNA Damage Assay Kit ab211154
ab211154 has been used in a variety of sample type including:
- Mouse liver samples (1)
- Rat liver and kidney tissues (2)
- HEK293FT cell line (3)
References:
1 - Song Y et al. 2023; 2 - Mesnage R et al. 2021; 3 - Kim D et al. 2021.
Related and recommended products
DNA damage Assay Kit requires purification of genomic DNA. We recommend using Genomic DNA Isolation Kit
ab65358
Another kit to study DNA damage is CPD ELISA Kit (cyclobutane pyrimidine dimers, DNA samples)
ab242297

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols