Abcam, ab211270, Anti-PUS3 antibody - C-terminal

Size: 100µg
Rabbit Polyclonal PUS3 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PUS3 aa 400 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PUS3 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9BZE2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab211270 was affinity purified using an epitope specific to PUS3 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pseudouridine synthase 3 (PUS3) also known as PUS3 homolog is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. PUS3 has a molecular mass of approximately 54 kDa. This enzyme is primarily located in the nucleoplasm but shows expression in diverse tissues with higher levels observed in the brain and testis. Its role is foundational in modifying RNA substrates impacting the stability and function of RNA.
Biological function summary
PUS3 contributes to essential RNA modifications important for accurate RNA structure and function. It engages in the pseudouridylation of specific RNA molecules including tRNA mRNA and spliceosomal RNAs. PUS3 functions mainly as a monomer and does not typically form part of larger enzyme complexes. The action of PUS3 enhances the stability and translational efficiency of RNA by altering standard nucleosides to pseudouridines influencing the tertiary structure of RNA molecules.
Pathways
PUS3 activity is essential for RNA modification pathways particularly those involving tRNA and mRNA processing. Within these pathways PUS3 cooperates with other pseudouridine synthases and RNA modifying proteins to achieve its biological role. It interacts indirectly with proteins like Dyskerin involved in similar RNA modifications highlighting the network of proteins necessary for proper RNA processing.
PUS3 mutations have been linked to developmental disorders such as intellectual disability. Disruptions in PUS3 lead to improper RNA modification patterns impacting gene expression and neural function. Notably PUS3's altered activity also connects to disorders involving defective RNA maturation where proteins like EXOSC8 part of the RNA exosome share pathways with PUS3 illustrating a relationship between erroneous RNA processing and disease outcomes.