Abcam, ab211754, Anti-Bag1 antibody [Y166] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal BAG1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y166,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).

Product details:
ab211754 is the carrier-free version of
ab32109
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bag1 protein also known as Bcl-2-associated athanogene 1 has a molecular mass of approximately 46 kDa. The protein is ubiquitously expressed in various tissues and cells indicating its broad functional significance. Bag1 serves as a co-chaperone interacting directly with heat shock protein 70 (Hsp70) to modulate its activity. This interaction affects the protein folding process ensuring cellular proteins maintain their proper conformation.
Biological function summary
Bag1 plays essential roles in regulating cell survival and apoptosis. It can exist as part of a larger protein complex and is known to bind with the anti-apoptotic protein Bcl-2 enhancing cell survival. By interacting with these proteins Bag1 helps control the delicate balance between cell death and survival critical for normal cellular function and response to stress. Bag1 also affects transcription regulation cell cycle progression and cellular response to stress stimuli.
Pathways
Bag1 integrates into cellular stress response and apoptotic signaling pathways. Bag1's interaction with Hsp70 associates it with the heat shock response pathway which upregulates molecular chaperones to protect cells during stress. Additionally its binding to Bcl-2 situates it within the apoptotic pathway where it supports cell survival by inhibiting caspase activation. The protein further interacts with key regulatory proteins like Raf-1 linking it to the MAPK/ERK signaling pathway involved in cell growth and division.
Dysregulation of Bag1 connects to cancer development and neurodegenerative diseases. Overexpression of Bag1 has associations with cancers such as breast cancer where it promotes tumor growth by inhibiting apoptosis. Bag1's ability to interact with the Bcl-2 protein makes it a significant player in cancer pathways. Additionally changes in Bag1 expression and function have been observed in Alzheimer's disease highlighting its role in neuron survival and stress response. Bag1's involvement with proteins like Hsp70 and Bcl-2 highlights its potential as a therapeutic target in these conditions.