Abcam, ab21176, Anti-Firefly Luciferase antibody

Size: 100µL
Anti-Firefly Luciferase antibody (ab21176) is a rabbit polyclonal antibody detecting Firefly Luciferase in Western Blot, ICC/IF . Suitable for Firefly . - Over 80 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Firefly,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Firefly Luciferin 4-monooxygenase.P08659

Product details:
Anti-Firefly Luciferase antibody (ab21176) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in human, mouse samples.
Anti-Firefly Luciferase antibody (ab21176) has been cited over 80 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Firefly Luciferase antibody (ab21176) has high sensitivity and specificity.
Anti-Firefly Luciferase antibody (ab21176) has 18 independent reviews from customers.
Anti-Firefly Luciferase antibody (ab21176) specifically detects Firefly Luciferase (UniProt ID: P13129; Molecular weight: 60kDa) and is sold in 100 µL selling sizes.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.
Biological function summary
Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.
Pathways
Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.
Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.