Abcam, ab212011, Rap1 Activation Assay Kit

Size: 20Test
Rap1 Activation Assay Kit (ab212011) uses RalGDS RBD Agarose beads to selectively isolate and pull-down the active form of Rap from purified samples or endogenous lysates.
Key facts
Sample types:Tissue, Suspension cells, Adherent cells,
Reacts with:Mouse, Rat, Human

Product details:
Rap1 Activation Assay Kit (ab212011) uses RalGDS RBD Agarose beads to selectively isolate and pull-down the active form of Rap from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rap is detected by western blot analysis using an anti-Rap1 polyclonal antibody, which reacts with the human, mouse and rat Rap1A and Rap1B proteins.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Rap1 (Ras-related protein 1), a 21 kDa protein of the Ras superfamily, exhibits 60% identity to Rap2. Rap2 regulates a variety of biological response pathways that include cell migration, cell adhesion, and embryonic blood vessel formation. Like other small GTPases, Rap1 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rap1 bind specifically to the Rap-binding domain (RBD) of RalGDS to control downstream signaling cascades.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C