Abcam, ab212053, Anti-Ankyrin erythroid/ANK antibody [S388A-10]

Size: 100µg
Mouse Monoclonal Ankyrin erythroid/ANK antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANK1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:S388A-10,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human ANK1.P16157,
Specificity:Does not cross-react with Ankyrin-B or Ankyrin-G.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ankyrin erythroid also known as ANK Ankyrin-R or Ankyrin-1 is a protein with a molecular mass of approximately 215 kDa. This protein is part of the ankyrin family which functions to help maintain cell structure and organization. Ankyrin erythroid is primarily expressed in erythrocytes where it plays an important role in connecting integral membrane proteins to the underlying spectrin-actin cytoskeleton. Its binding capability and mechanical support are essential for the stability and elasticity of the red blood cell membrane.
Biological function summary
Ankyrin erythroid acts as a linker protein and contributes to the structural integrity of the erythrocyte membrane. It is an important component of the ankyrin-spectrin complex which provides necessary mechanical support to the cell's lipid bilayer. The stability offered by this complex is key for erythrocytes' remarkable ability to withstand deformation as they transit through narrow capillaries. Ankyrin erythroid interacts with multiple proteins including band 3 and spectrin supporting cellular health and longevity by maintaining membrane cohesion and surface area.
Pathways
Ankyrin erythroid plays a significant role in the cytoskeletal network and the erythrocyte membrane stabilization pathways. Its interaction with band 3 and spectrin is central in the maintenance of cell surface protein organization and ion transport pathways. These interactions and the structural framework have direct implications on red blood cell function and homeostasis. Ankyrin erythroid's pathway relations emphasize its part in ensuring the flexibility and stability of erythrocyte membranes through its role in maintaining the organization and stability of the cytoskeleton.
Defects or deficiencies in ankyrin erythroid can lead to hereditary spherocytosis an anemia characterized by the presence of spherical erythrocytes that are prone to hemolysis. This condition sees disruptions in the ankyrin-spectrin complex affecting cell elasticity and lifespan. Additionally ankyrin erythroid interacts with proteins like spectrin and band 3 where mutations can exacerbate membrane stability issues further contributing to the pathology of hereditary spherocytosis. Understanding these interactions provides insight into potential therapeutic interventions for related hematological disorders.