Abcam, ab213181, Anti-DEPDC5 antibody [EPR20497-23]

Size: 20µL / 100µL / 1mL
Anti-DEPDC5 antibody [EPR20497-23] (ab213181) is a rabbit monoclonal antibody detecting DEPDC5 in Western Blot . Suitable for Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20497-23,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-DEPDC5 antibody [EPR20497-23] (ab213181) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.
What is the molecular weight of DEPDC5?
Anti-DEPDC5 [EPR20497-23] (ab213181) specifically detects a band for DEPDC5 (UniProt: O75140) at a molecular weight of 181kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-DEPDC5 antibody [EPR20497-23] (ab213181) has been confirmed by Western blot testing in DEPDC5 Knockout A549 cell line,
ab266906
Other related products
We have a range of other formats of antibody clone [EPR20497-23] also available for your convenience: ab213181, Carrier free -
ab272399
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DEPDC5 also called DEP domain containing 5 is a protein that plays a significant role in various cellular processes. It belongs to a protein family known for its involvement in signal transduction. DEPDC5 has a mass of about 160 kDa. This protein expresses broadly in many tissues with notable presence in the brain and the liver indicating its potential importance in these organs.
Biological function summary
DEPDC5 acts as a negative regulator of the mTORC1 signaling pathway by being a part of the GATOR1 complex. This complex is involved in nutrient sensing and regulation of cell growth and metabolism. DEPDC5's role in mTORC1 regulation links it to the control of protein synthesis and autophagy essential processes in maintaining cellular homeostasis. By interacting with other components of the GATOR1 complex DEPDC5 contributes not only to inhibiting mTORC1 activity but also to the broader metabolic balance.
Pathways
DEPDC5 is integral to the mTOR signaling pathway and the amino acid sensing pathways. The protein acts in coordination with other GATOR1 complex components such as NPRL2 and NPRL3 which together monitor nutrient availability and adapt cellular functions accordingly. These pathways highlight DEPDC5's role in connecting nutrient signals to cellular growth responses showing its influence on critical regulatory networks affecting cell growth and proliferation.
Mutations in DEPDC5 have been linked to certain neurological disorders notably focal epilepsy. The protein's involvement in the brain points to its connection with proper neural function where its disruption may lead to disease. Researchers have also found related proteins like MTOR and NPRL3 being associated in these epilepsy cases emphasizing the importance of DEPDC5 in neural signaling pathways and its potential as a target for therapeutic approaches.