Abcam, ab213369, Anti-Tet2 antibody [EPR20546-135]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal TET2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20546-135,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tet2 also known as ten-eleven translocation methylcytosine dioxygenase 2 is an enzyme involved in DNA demethylation. It has a molecular weight of approximately 224 kDa. This enzyme catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine an important step in DNA demethylation processes. Tet2 expression occurs in various tissues with higher levels in hematopoietic cells. Its role in modification of DNA has made it a significant subject of study in epigenetic regulation.
Biological function summary
Tet2 participates in the regulation of gene expression through its impact on DNA methylation status. By promoting DNA demethylation Tet2 influences transcriptional networks that are vital for cell differentiation and development. The enzyme functions as part of a larger complex with cofactors including ascorbic acid which it requires for full enzymatic activity. Its actions affect the regulation of important processes such as self-renewal and differentiation in hematopoietic stem cells.
Pathways
The enzymatic activity of Tet2 plays a role in epigenetic pathways involved in hematopoiesis and immune function. In particular Tet2 interacts with pathways regulating chromatin structure and transcriptional control. It associates with proteins like TET1 and TET3 as they share roles in the regulation of 5-hydroxymethylcytosine levels. Through these mechanisms Tet2 contributes significantly to the maintenance of normal hematopoietic and immune cell function.
Alterations in Tet2 activity have been linked to hematological malignancies such as myelodysplastic syndromes and acute myeloid leukemia. Loss of function mutations in Tet2 lead to disruptions in hematopoietic differentiation contributing to disease pathology. Tet2 mutations can affect interactions with proteins such as DNMT3A another promoter of DNA methylation which further influence the disease course. Understanding Tet2's role in these disorders may offer potential therapeutic insights.