Abcam, ab213474, Mouse TARC ELISA Kit (CCL17)

Size: 1 x 96Tests
Mouse TARC ELISA Kit (CCL17) is a single-wash 90-min Simplestep used to quantify Mouse TARC (CCL17) with a sensitivity of 2.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.5 pg/mL,
Range:7.8 - 500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse TARC ELISA Kit (CCL17) ab213474 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse TARC (CCL17)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.5 pg/mL.
How the assay works
Mouse TARC (CCL17)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse TARC ELISA Kit (CCL17) ab213474 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
TARC (also known as C-C motif chemokine 17) is a chemokine that functions through chemokine receptors CCR4 and CCR8. TARC is expressed in the thymus as well as by dendritic cells, keratinocytes and bronchial epithelial cells. The standard is affinity purified.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TARC also known as CCL17 is a chemokine with a molecular mass of approximately 8 kDa. It is mainly expressed in the thymus but also found in the lung liver and lymph nodes. CCL17 plays an important role in immune response by signaling through the CCR4 receptor. It functions as a ligand that guides the movement of T-helper 2 (Th2) cells regulatory T cells (Tregs) and other immune cells to sites of inflammation.
Biological function summary
CCL17 acts as a chemotactic agent attracting specific immune cells especially T lymphocytes to inflammatory regions. This protein does not operate as part of a larger protein complex but engages directly by binding to receptors on the target immune cells. Localized expression of CCL17 increases in conditions where enhanced Th2 cell response is needed such as allergic or autoimmune responses.
Pathways
CCL17 plays a significant role in the cell signaling pathway called the chemokine signaling pathway. Through this pathway CCL17 interacts with the CCR4 receptor which helps mediate immune cell migration and adhesion. This interaction is critical for establishing the adaptive immune response. CCL17 and CCR4 support the trafficking of Th2 cells during immune reactions and pathogen defense.
CCL17 associates with asthma and atopic dermatitis where Th2 cell recruitment and activity are heightened. In asthma increased CCL17 levels exacerbate inflammatory response in the airways. In atopic dermatitis CCL17 presence corresponds with the intensity of the skin inflammation. These conditions are linked to the overactivity of Th2 cytokines such as IL-4 (H4L3) which collaborate with CCL17 to drive allergic responses.