Abcam, ab213777, Human FCN1/M-Ficolin ELISA Kit

Size: 1 x 96Tests
Human FCN1/M-Ficolin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human FCN1/M-Ficolin in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:0.78 - 50 ng/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human FCN1/M-Ficolin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213777) is designed for the quantitative measurement of Human FCN1/M-Ficolin in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FCN1/M-Ficolin has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A30-A326) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FCN1/M-Ficolin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FCN1/M-Ficolin amount of sample captured in plate.
FCN1/M-Ficolin, also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. FCN1/M-Ficolin is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FCN1 also known as M-Ficolin or ficolin-1 is a pattern recognition molecule part of the innate immune system. It plays a role in recognizing pathogen-associated molecular patterns (PAMPs) on the surface of microbes. FCN1 is a 35 kDa protein predominantly expressed in the liver and secreted into the bloodstream. It consists of a collagen-like domain and a fibrinogen-like recognition domain allowing it to effectively bind its targets. The protein occurs in plasma where it particularly acts in host defense mechanisms.
Biological function summary
FCN1 functions as part of the body’s first line of immune defense by binding to microbial surfaces facilitating the opsonization and activation of the lectin complement pathway. It is a member of a complex known as the lectin pathway of complement activation which includes MASP1 and MASP2 proteins. FCN1 triggers this pathway when it recognizes patterns such as N-acetylglucosamine on the surface of pathogens which is important for initiating the immune response and promoting pathogen clearance through complement activation in cooperation with other components of the pathway.
Pathways
FCN1 primarily participates in the lectin complement pathway distinctively different from the classical and alternative pathways. It interacts with MASP proteins to trigger the cleavage of complement proteins which leads to a cascade that results in inflammation opsonization and pathogen lysis. FCN1 connections also extend to other pattern recognition receptors such as those in the toll-like receptor pathway which harmonize to ensure a robust immune response by recognizing wide array of pathogen motifs.
FCN1 involvement has been observed in several conditions notably systemic lupus erythematosus (SLE) and bacterial infections. Alterations or deficiencies in FCN1 levels can influence susceptibility to infections due to compromised opsonization and pathogen clearance. In SLE FCN1 and its associated pathway proteins may contribute to the disease pathogenesis by affecting complement system activity leading to autoimmune and inflammatory responses. Understanding these associations aids in unraveling the complex network of immune response regulation and disease development.