Abcam, ab213815, Human PEDF ELISA Kit (SerpinF1)

Size: 1 x 96Tests
Human PEDF ELISA Kit (SerpinF1) is a sandwich ELISA designed to quantify Human PEDF (SerpinF1) with a sensitivity of 20 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.78 - 50 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:0.78 - 50 ng/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human PEDF ELISA Kit (SerpinF1) ab213815 is a sandwich ELISA to measure Human PEDF (SerpinF1) in serum, EDTA plasma, heparin plasma, cell culture supernatant, cell lysate with a sensitivity of 20 pg/ml.
How the assay works
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for PEDF has been pre-coated onto 96-well plates. Standards (NSO, M1-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PEDF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PEDF amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human PEDF ELISA Kit (SerpinF1) ab213815 protocol summary
1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm
Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, anti-tumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the anti-tumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.
Biological function summary
PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.
Pathways
PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.
PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.