Abcam, ab213841, Human TNFSF11 ELISA Kit (RANKL)

Size: 1 x 96Tests
Human TNFSF11 ELISA Kit (RANKL) is a sandwich ELISA designed to quantify Human TNFSF11 (RANKL) with a sensitivity of 10 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 78 - 5000 pg/mL - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:78 - 5000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human TNFSF11 ELISA Kit (RANKL) ab213841 is a sandwich ELISA to measure Human TNFSF11 (RANKL) in serum, EDTA plasma, heparin plasma, cell culture supernatant, cell lysate with a sensitivity of 10 pg/ml.
How the assay works
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for TNFSF11/RANKL has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: G64-D245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF11/RANKL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFSF11/RANKL amount of sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human TNFSF11 ELISA Kit (RANKL) ab213841 protocol summary
1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome 13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C