Abcam, ab214170, Anti-ERK2 antibody [E460] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ERK2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E460,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:ab32081 reacts with an epitope located in the C terminal region of ERK2.

Product details:
ab214170 is the carrier-free version of
ab32081
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERK2 also known as Extracellular signal-Regulated Kinase 2 is a serine/threonine protein kinase in the mitogen-activated protein kinase (MAPK) family with a mass of approximately 42 kDa. This kinase is expressed in many cell types and tissues including the brain liver and lungs. ERK2 plays a significant role in cellular processes such as proliferation differentiation and survival. It is often analyzed using specific assays including ERK2 ELISA and examination of cell lysate samples to determine expression levels and activity.
Biological function summary
ERK2 influences several key cellular functions. It functions as part of a signaling cascade transmitting signals from the exterior to the cell nucleus. In this cascade ERK2 is often part of a multi-protein complex that undergoes sequential phosphorylation. Through these mechanisms ERK2 regulates gene expression and is a pivotal component of the MAPK/ERK pathway ensuring the proper response to growth signals and stress stimuli.
Pathways
ERK2 is a central component of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways play critical roles in cell cycle regulation and apoptosis. ERK2 activation leads to its interaction with the MEK1/2 proteins which further allows the transmission of mitogenic signals. The interplay between ERK2 and related proteins like E460 often impacts cellular growth and development as it precisely controls the phosphorylation events within the pathway.
ERK2 is connected to certain types of cancer and neurodegenerative diseases. Aberrations in ERK2 signaling pathways are often linked to tumorigenesis where altered interaction with proteins such as Raf and MEK1/2 disrupts cell cycle regulation and apoptosis. In neurodegenerative disorders dysregulated ERK2 activity has been associated with proteins contributing to Alzheimer’s disease indicating its involvement in neuronal survival and stress response mechanisms.