Abcam, ab214192, Anti-epithelial Sodium Channel alpha antibody

Size: 100µL
Rabbit Polyclonal epithelial Sodium Channel alpha antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCNN1A aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SCNN1A aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P37088

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The epithelial sodium channel alpha also known as the alpha subunit of the epithelial sodium channel (ENaC) is an important component of the ENaC channels. This protein forms part of the larger ENaC complex and plays an important role in sodium ion transport across epithelial cells. Typically the ENaC channel has a molecular mass of approximately 70 to 110 kDa. The channel is expressed in epithelial tissues such as the kidney lung and colon where it acts to regulate sodium excretion and reabsorption.
Biological function summary
The epithelial sodium channel alpha facilitates sodium ion entry into cells which impacts fluid balance blood pressure and extracellular fluid volume. The alpha subunit functions as part of a heterotrimeric complex consisting of alpha beta and gamma subunits forming the complete active channel. The coordinated action of these subunits ensures regulated ion transport which is essential for maintaining homeostasis in epithelial tissues.
Pathways
ENaC channels participate in the renin-angiotensin-aldosterone system and are involved in sodium reabsorption processes. This channel works closely with proteins like aldosterone to modulate its activity in response to changes in body fluid status. ENaC's ability to regulate sodium flux links it to key physiological functions in fluid and electrolyte balance pathways.
Alterations in ENaC function are linked to conditions such as Liddle's syndrome and cystic fibrosis. Liddle's syndrome involves mutations that lead to excessive sodium reabsorption resulting in hypertension. Furthermore ENaC function is disrupted in cystic fibrosis due to altered protein interactions contributing to imbalanced mucus composition. Proteins like cystic fibrosis transmembrane conductance regulator (CFTR) interact with ENaC in cystic fibrosis illustrating their interconnected role in disease pathology.