Abcam, ab214728, Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236]

Size: 50µg
Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (ab214728) is a rabbit monoclonal antibody detecting 5-hydroxymethylcytosine (5-hmC) in Flow Cytometry, IP, IHC-P, ICC/IF, ELISA, Dot Blot . - Recombinant format for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:RM236,
Isotype:IgG,
Carrier free:No,
Applications:MeDIP, ICC/IF, Dot, IHC-P, Flow Cyt, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ab214728 reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Product details:
What is this antibody validated in?
Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (ab214728) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot.
Trusted by the scientific community
Anti-5-hydroxymethylcytosine (5-hmC) [RM236] (ab214728) was first used in a scientific publication in 2016 and has been cited over 20 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [RM236] also available for your convenience: ab214728, ab319976, Carrier free - ab323582

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
5-hydroxymethylcytosine (5-hmC) also known as hydroxymethylcytosine represents a significant epigenetic modification in DNA. This target with a mass of approximately 63.3 emerges through DNA demethylation processes where ten-eleven translocation (TET) enzymes oxidize 5-methylcytosine (5-mC). It is found largely in the brain where it shows high levels of expression but it can also appear in other tissues to a lesser extent. The modification is important in regulating gene expression and maintaining cellular identity.
Biological function summary
5-hydroxymethylcytosine plays a critical role in epigenetic regulation and serves as an intermediary in the active DNA demethylation process. It is not part of a stable complex but interacts transiently during epigenetic reconfiguration. The presence of 5-hmC marks regulatory regions of genes and is involved in transcriptional activation or repression depending on the cellular context. Its levels are dynamic influenced by various developmental stages and environmental conditions.
Pathways
This DNA modification is an important player in pathways related to cellular differentiation and development. It is integrally involved in the DNA demethylation pathway where TET enzymes mediate its formation from 5-methylcytosine. This pathway also involves other modifications leading to cytosine turnover linking 5-hmC to broader chromatin remodeling processes. Proteins interacting with 5-hmC include TET enzymes and other DNA maintenance proteins which highlight its role in maintaining genomic stability.
Aberrant 5-hmC levels show connection to several pathological states including cancer and neurological disorders. Altered hydroxymethylation patterns often associate with tumorigenesis particularly in gliomas where the misregulation of 5-hmC accompanies cancer progression. Neurological disorders like Rett syndrome also exhibit altered levels of 5-hmC implicating its role in neurodevelopmental processes. In these diseases mutations in proteins such as TET enzymes link to disrupted 5-hmC levels indicating its critical involvement in both oncogenesis and neurodevelopmental regulation.