Abcam, ab215191, Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal

Size: 100µL / 1mL
Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) is a rabbit monoclonal antibody detecting DFNA5/GSDME in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP . Suitable for Human, Mouse, Rat . - Clone EPR19859 is the most cited clone to DFNA5/GSDME - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 150 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19859,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Our previous testing data indicates that this antibody detects no signal or very weak signal in mouse heart, spleen, lung, stomach, testicle, skin, pancreas, muscle and lymph node tissues. Final results may be affected by the expression level of the tested samples.

Product details:
Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), IP and WB.
Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) was first used in a scientific publication in 2017 and has been cited over 153 times in peer reviewed journals. It's performance in Western blot in mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) has been confirmed by Western Blot testing in DFNA5/GSDME knockout SGC-7901 cells.
Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) has 4 independent reviews from customers.
Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) specifically detects DFNA5/GSDME (UniProt ID: O60443; Molecular weight: 55kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR19859 -
ab223877
Antibody clone EPR19859 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, PE (
ab225519
ab225520
Gasdermin E (GSDME) plays a significant role in immunology by modulating the immune response, particularly through its involvement in pyroptosis, a form of programmed cell death
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DFNA5 also known as GSDME (Gasdermin E) is a protein encoded by the GSDME gene. It has a molecular mass of approximately 59 kDa. The protein is expressed in various tissues including the cochlea in the inner ear and some epithelial tissues. Mechanically DFNA5/GSDME plays a role in inducing cell membrane pore formation leading to cell lysis. This activity relates to its involvement in processes like cell death specifically pyroptosis where the cell undergoes a form of programmed necrosis.
Biological function summary
DFNA5/GSDME serves as a pore-forming protein that facilitates pyroptotic cell death usually upon cleavage by caspase-3. This activity is important for innate immune response and the maintenance of cellular homeostasis. DFNA5/GSDME operates independently and does not form part of large protein complexes. The cleaved form inserts into the lipid bilayer of cell membranes contributing to the execution of cell death particularly under stress conditions or cellular insult.
Pathways
DFNA5/GSDME is involved in the pyroptosis and apoptosis pathways. It plays an essential role in the cellular response to inflammation and stress signals by interacting with caspase-3 a critical protease in the apoptosis pathway. The balance between apoptosis and pyroptosis decides cell fate with DFNA5/GSDME activation tipping towards pyroptotic cell death. The interconnection with other gasdermin family proteins such as GSDMD is notable as they share functional and structural similarities in promoting necrosis-like cell death processes.
Mutations in the DFNA5 gene relate to progressive hearing loss (nonsyndromic sensorineural deafness). Dysregulation of GSDME has also been implicated in cancer where altered expression promotes tumorigenesis or tumor suppression depending on the context. In hearing loss GSDME's interaction with pathways influencing apoptosis highlights its pathogenic role while in cancer connections to caspase-3 show a potential dichotomy in cell survival and cell death regulation.