Abcam, ab215263, Anti-MEK1 + MEK2 antibody [EPR16667] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MEK1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16667,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IP, WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab215263 is the carrier-free version of
ab178876
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MEK1 and MEK2 also known as MAP2K1 and MAP2K2 respectively are dual-specificity kinases that play roles in signaling pathways mediating cellular responses. MEK1 and MEK2 have molecular weights of approximately 45kDa. They are expressed in various tissues including the heart brain and skeletal muscles reflecting their widespread function within the organism. These proteins are often referred collectively as the MEK1/2 complex due to their shared and overlapping roles in cell signaling.
Biological function summary
MEK1 and MEK2 are integral parts of the mitogen-activated protein kinase (MAPK) signaling cascade. MEK1/2 phosphorylate and activate the ERK1/2 kinases which transmit signals from the cell surface to the nucleus regulating gene expression and cell fate decisions. The MEK1/2 proteins also act in concert with other kinases and substrates forming signaling complexes that ensure specific and regulated cellular outcomes.
Pathways
MEK1 and MEK2 play key roles within the MAPK/ERK pathway a critical signaling mechanism for cell division and survival. They also link to the RAS/MAPK pathway involved in transmitting signals from membrane receptors to the nucleus. In these pathways MEK1/2 interact with proteins like RAF kinases and ERK1/2 propagating signals that influence cellular growth and differentiation.
MEK1 and MEK2 are implicated in cancer and neurological disorders. Abnormal activity of the MEK1/2 proteins often due to mutations can lead to uncontrolled cell proliferation contributing to the development of cancers such as melanoma. In neurological disorders dysregulation of MEK1/2 signaling relates to conditions like neuro-cardio-facial-cutaneous syndromes. These proteins interact with other key players in these diseases such as mutant RAF proteins in melanoma highlighting their critical roles in disease mechanisms.